Ms IL-12 p70 Recom 5ug

产品编号：BD-554592 品牌：BD-Pharmingen 原厂货号：554592
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-12 Family 白细胞介素-12超家族
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 小鼠
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

5ug

运保温度:

Store product at -80°C prior to use or for long te

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描述：

Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can; i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells; ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells; iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and; iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Formulation and Purity:

The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 μm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 μg BSA to 1 μg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law. The endotoxin level is ≤ 0.1 ng per μg of mouse IL-12, as measured in a chromogenic LAL assay.

Preparation and Storage

Store product at -80°C prior to use or for long term storage of stock solutions.

For long term storage of lyophilized vials or stock solutions, store at –80°C. Lyophilized IL-12 can be reconstituted to 50 μg/ml with sterile water. To minimize possible loss, the buffer should be added directly through the vial rubber stopper using a needle syringe. The product can be diluted in sterile neutral buffer containing no less than 1 to 10 mg/ml carrier protein such as human or bovine albumin, aliquoted into polypropylene microtubes and frozen at –80°C for future use. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity. For in vitro biological assay use, we recommend carrier-protein concentrations of 1 - 2 mg/ml. For use as an ELISA standard we recommend carrierprotein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 50 μg/ml for long term storage.Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
For long term storage of lyophilized vials or stock solutions, store at –80°C. Lyophilized IL-12 can be reconstituted to 50 μg/ml
with sterile water. To minimize possible loss, the buffer should be added directly through the vial rubber stopper using a needle
syringe. The product can be diluted in sterile neutral buffer containing no less than 1 to 10 mg/ml carrier protein such as human
or bovine albumin, aliquoted into polypropylene microtubes and frozen at –80°C for future use. Carrier proteins should be
pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to
toxicity, high endotoxin levels or possible blocking activity. For in vitro biological assay use, we recommend carrier-protein
concentrations of 1 - 2 mg/ml. For use as an ELISA standard we recommend carrierprotein concentrations of 5 - 10 mg/ml.
Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be
diluted to less than 50 μg/ml for long term storage.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may
effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.