描述:
Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) reacts with human interleukin-7 (IL-7). This antibody can be useful as a
detection antibody for sandwich ELISA measuring human IL-7 protein levels. Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10)
can be paired with Purified Rat Anti-Human IL-7 antibody (clone BVD10-40F6; Cat. No. 554493) as the capture antibody along with
recombinant human IL-7 (Cat. No. 554608) as the standard. Investigators are advised to titrate to determine optimal usage concentrations
(ELISA), with a suggested range of 2 ug/mL to 0.5 ug/mL for Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10). To obtain linear
standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate.
This ELISA pairing has been reported to demonstrate limited to no cross-reactivity with the following cytokines: Mouse IL-1β, IL-2, IL-3,
IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF or Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5,
IL-6, IL-8, IL- 9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α,
MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF.