p53 FITC Set DO-7 100Tst

• 产品编号：BD-554298      品牌：BD-Pharmingen       原厂货号：554298
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

p53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA
damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of
the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and
wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human
papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is
still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins.
DO-7 recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7
recognizes an epitope between amino acids 1-45 of the known forms of human p53. Human recombinant p53 protein was used as immunogen.
Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies in normal tissues. Clone DO-7 was
initially characterized by immunoprecipitation, western blot analysis, immunohistochemistry of frozen and formalin-fixed paraffin-embedded
tissue sections, and flow cytometric analysis.
Clone 27-35 is an isotype control monoclonal antibody specific for hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is
not expressed on human cells or human cell lines. The DO-7 and 27-35 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was
experimentally determined by flow cytometric analysis.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.