Ms Bcl-2 FITC Set 3F11 100Tst

• 产品编号：BD-554221      品牌：BD-Pharmingen       原厂货号：554221
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenence of
tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane
such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In
the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting
significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the cellular response to
apoptotic signals. Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types. Bcl-2 is thought to provide
selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kD intracellular, integral
membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.
Clone 3F11 reacts with mouse Bcl-2. It does not cross-react with human Bcl-2. Gel purified recombinant mouse Bcl-2 protein was used as
immunogen.
Clone A19-3 is an Armenian hamster IgG isotype control which is specific for the hapten trinitrophenol (TNP). This hapten is not expressed
on human cells or human cell lines. The solutions are free of unconjugated antibody. The 3F11 and A19-3 FITC conjugates are matched in F/P
ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.