Rat/Ham Ig Kpa Comp Bead Set 6mL

• 产品编号：BD-552845      品牌：BD-Pharmingen       原厂货号：552845
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 微球 > 荧光补偿微球
• 应用分类：

描述：

The BD™ CompBeads Compensation Particles Anti-Rat/Hamster Ig, κ Set are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD CompBeads Anti-Rat/Hamster Ig, κ particles, which bind any rat or hamster κ light chain-bearing immunoglobulin, and the BD CompBeads Negative Control (FBS) Particles, which has no binding capacity. When mixed together with a fluorochrome-conjugated rat or hamster antibody, the BD CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Note: BD Horizon™ V500 and AmCyan conjugated reagents can show significant differences in emission spectrum on stained cells and when captured on BD™ CompBeads. Thus, spillover values for these dyes evaluated with BD™ CompBeads may not provide correct compensation for cells. Therefore, single stained cellular controls are recommended to set up compensation for AmCyan and BD Horizon™ V500 reagents. BD Horizon™ V500-C has been modified to minimize these spectral differences and BD™ CompBeads may be used to determine spillover values for RUO antibodies conjugated to BD Horizon™ V500-C.

原厂资料：

The BD™ CompBeads Compensation Particles Anti-Rat/Hamster Ig, κ Set are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD CompBeads Anti-Rat/Hamster Ig, κ particles, which bind any rat or hamster κ light chain-bearing immunoglobulin, and the BD CompBeads Negative Control (FBS) Particles, which has no binding capacity. When mixed together with a fluorochrome-conjugated rat or hamster antibody, the BD CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Note: BD Horizon™ V500 and AmCyan conjugated reagents can show significant differences in emission spectrum on stained cells and when captured on BD™ CompBeads. Thus, spillover values for these dyes evaluated with BD™ CompBeads may not provide correct compensation for cells. Therefore, single stained cellular controls are recommended to set up compensation for AmCyan and BD Horizon™ V500 reagents. BD Horizon™ V500-C has been modified to minimize these spectral differences and BD™ CompBeads may be used to determine spillover values for RUO antibodies conjugated to BD Horizon™ V500-C.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.