BrdU In-Situ Dtec Kit II

• 产品编号：BD-551321      品牌：BD-Pharmingen       原厂货号：551321
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 二级检测试剂盒 > Enzyme Amplification Kits & Reagents
• 应用分类：

原厂资料：

Cell proliferation is an important and highly studied cellular function. Different approaches to measurement of proliferation include the identification of mitotic figures and the use of autoradiography to identify [3H]-thymidine incorporation into DNA by proliferating cells.1 In 1982, Gratzner et al. reported an approach to the detection of cells in
S (synthetic)-phase of cell cycle, based on the use of antibodies against bromodeoxyuridine (BrdU), a thymidine analog.2 Cellular incorporation of BrdU during S phase can be readily detected by anti-BrdU-specific antibodies following membrane permeabilization. Subsequent quantitation of cells positive for BrdU is possible using routine methods for cell analysis such as flow cytometry or immunohistochemistry.
One advantage of the immunohistochemical detection of BrdU compared to [3H]-thymidine incorporation and subsequent autoradiography is that this approach takes much less time and provides high resolution.3-5 Immunohistochemical staining for incorporated BrdU has been used to determine proliferation rates and cell cycle kinetics by measuring the labeling indices of different cell populations in normal and cancerous tissues.6-9
The most common technical difficulty in obtaining effective staining for BrdU is the inability of the antibody to penetrate between the DNA strands. Typically, methods such as treatment with HCl, ethanol fixation, enzymatic digestion, or microwave pre-treatment are used to permeabilize the cell and relax the DNA to make the BrdU accessible to the antibody. Unfortunately, treatment with HCl or enzymatic digestion destroys architectural detail and more importantly reduces or destroys immunoreactivity to other antigens. To mitigate this problem and improve immunostaining, alternative fixatives such as methacarn or antigen retrieval techniques using heat and citrate buffer, can be employed to unmask the antigenic sites leading to improved immunostaining. Use of these antigen retrieval methods are also compatible with simultaneous detection of other antigens.10-14

注意事项：

All the components of the BrdU kit must be stored at 4°C. Some solutions require diluting or mixing prior to use. Instructions for handling, preparation, and storage of kit components follow: