Rat Ig HRP Dtec Kit

• 产品编号：BD-551013      品牌：BD-Pharmingen       原厂货号：551013
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 二级检测试剂盒 > Enzyme Amplification Kits & Reagents
• 应用分类：

原厂资料：

Immunohistochemistry is a powerful technique that allows a researcher to detect
molecules within a tissue microenvironment. Protein expression can be studied in
relation to tissue or cell compartments, and changes due to either normal or pathologic
stimuli can be evaluated. Detection of an antibody bound to antigen can be
accomplished either by direct or indirect methods. The direct method uses a primary
antibody labeled with a fluorescent tag or linked with an enzyme. The indirect
method uses an enzyme-linked secondary antibody that binds to the primary
antibody. For even greater sensitivity, a biotinylated secondary antibody is used
with a tertiary step such as an enzyme-linked avidin or streptavidin. While the
indirect methods involve more assay steps, the amplification process at each step
leads to increased sensitivity.
Although very simple in principle, immunohistochemistry involves many steps such
as proper fixation of tissue, tissue processing, and sectioning. Antigen retrieval is
often required for optimal antigen detection. Each of these steps are crucial for
optimal immunostaining and for maintaining good tissue morphology, both of which
are important to understand the biological process in situ. The direct method uses
a primary antibody labeled with a fluorescent tag or an enzyme.

注意事项：

1. Label base mold and partially fill the mold with frozen tissue matrix.
2. Sacrifice animal by prescribed and approved euthanasia techniques.
3. Remove desired tissues, trim and cut tissue to no more than 5 mm in thickness.
Place in pre-labeled base molds filled with frozen tissue matrix. Arrange
tissue in the matrix near the bottom so tissue is easily exposed when
sections are cut.
4. Place a stainless steel beaker of 2-methylbutane in liquid nitrogen and allow
to cool adequately. Place base mold with tissue into the beaker of cold
2-methylbutane and quickly immerse the block. Allow the tissue matrix to
solidify completely and remove block from 2-methylbutane and place on
dry ice or in the –20°C cryostat.
Note: If block is left in 2-methylbutane too long, the block may crack.
5. Store blocks in the –80°C freezer until ready for sectioning.