The Jurkat Apoptotic Lysate Set I is designed to be used as a western blot positive control for detecting antigens expressed during apoptosis.
Jurkat cells in log-phase growth were either left untreated or treated with 4 μM camptothecin for 4 hours to induce apoptosis. The cells were
collected, lysed in lysis buffer [1% SDS, 1 mM sodium orthovanadate, 10 mM Tris (pH 8.0)], and aliquoted in SDS sample buffer.
注意事项:
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.2.Store undiluted at -20°C.
Do not dilute or boil.