BD Retrievagen B (pH 9.5) is an antigen retrieval system that is recommended for Zinc-fixed and Formalin-fixed paraffin embedded tissue
sections that show sub-optimal or no reactivity with antibodies for immunohistochemical staining. 10% neutral buffered formalin fixes the
tissue by cross-linking proteins that may cause a conformational change or "masking" of the antigenic epitope leading to reduced or absent
antibody reactivity. The BD Retrievagen B (pH 9.5) is designed to unmask the epitope and cause the enhancement of antibody staining. The
proposed mechanism by which most of the antigen retrieval systems work is through breaking the cross-linking bonds formed between the
protein molecules in the fixed tissue sections.
It is important to realize that in spite of antigen retrieval, some antibodies do not stain on formalin-fixed paraffin sections. In these situations
fixing the tissue in a less harsh fixative like the IHC Zinc fixative developed by BD Pharmingen (Cat. No. 550523) may be helpful. Our
Zinc fixative preserves the epitope leading to good antibody staining at the same time depicting good tissue morphology. However, there are
a few antigens that are not preserved by Zinc-fixation either and in these situations use of fresh frozen tissue for immunohistochemical
staining is the only alternative.
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Store undiluted at 4°C .
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