GenePrint ® 10 系统包含从人类基因组 DNA 扩增 STR 基因座所需要的各种组份,其中热启动 DNA 聚合酶已包含在 GenePrint ® 10 5X Master Mix 中。系统中提供内标( Internal lane standard , ILS )和等位基因 Ladder 用于标准化样品, 2800M Control DNA 为阳性对照。扩增后,将 ILS 加入每个样品,用于毛细管电泳以确定每个扩增产物的片段大小。等位基因 Ladder 定义了每个基因座中最常见的等位基因,用于正确标识每个等位基因的参照物。 GenePrint ® 10 等位基因 Ladder 的信息,包括每个等位基因的大小和重复次数可查阅技术手册。 2800M Control DNA 有已知的基因型,可用于验证基因分型的准确性。
• 基因型的自动分析: 利用 GeneMapper ® ID 和 ID-X 软件进行自动分型时需要 Panel 和 Bin 文件。可从网站上免费下载 Panel 和 Bin 文件。
原厂资料:
TheGenePrint® 10 System allows co-amplification and three-color detection of ten human loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317, D5S818 and D21S11) for human cell line authentication. These loci collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109and exceed standard ASN-0002 issued by the American Tissue Culture Collection Standards Development Organization Workgroup for cell line authentication. TheGenePrint® 10 System is compatible with the ABI PRISM® 3100 and 3100-AvantGenetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. The system contains all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. An internal lane standard (ILS) and allelic ladder are provided for sizing and genotyping of amplified fragments, and the 2800M Control DNA is supplied as a positive control.
注意事项:
收货后,将 2800M Control DNA 取出并储存于 4 ° C ,其余组份储存在 -20 ° C 。