OneTaq (Mg-Free) GC Reaction Buffer Pack

• 产品编号：B9025S      品牌：New England Biolabs       原厂货号：B9025S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
5X OneTaq (Mg-Free) GC Reaction Buffer and OneTaq High GC Enhancer are optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer, along with supplemental enhancer and MgSO₄ have been formulated for robust yields of difficult or GC-rich amplicons while allowing for maximal flexibility of Mg²⁺ optimization. The OneTaq (Mg-Free) GC Reaction Buffer is recommended for GC-rich (> 65% GC) amplicons. For particularly difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to

Reaction Conditions:
1X OneTaq (Mg-Free) GC Reaction Buffer, 1.5–2.0 mM MgSO₄, 10–20% OneTaq High GC Enhancer (optional), DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of OneTaq or OneTaq Hot Start DNA Polymerase (not included) in a total reaction volume of 50 µl.

Reagents Supplied:
5X OneTaq (Mg-Free) GC Reaction Buffer
MgSO₄ (100 mM)
OneTaq High GC Enhancer

1X OneTaq (Mg-Free) GC Reaction Buffer:
80 mM Tris-SO₄
20 mM (NH₄)₂SO₄
5 % Glycerol
5 % DMSO
0.06 % IGEPAL® CA-630
0.05 % Tween-20
pH 9.2 @ 25°C
 

OneTaq High GC Enhancer:
10 mM Tris-HCl
25% DMSO
25% Glycerol
pH 9.2 @ 25°C

原厂资料：