Description: This medium pack includes 12 grams of Yeast Carbon Base and 10 ml of Acetamide.
The Yeast Carbon Base (YCB) Medium Pack contains sufficient reagents needed to make 1 liter of YCB Agar Medium containing 5 mM acetamide.
YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K.lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
Reagents Supplied:
Yeast Carbon Base Medium 12 grams
100X acetamide solution (sterile) 10 ml
京公网安备11010802025653 版权所有：北京逸优科技有限公司
