ThermoPol II (Mg-free) Reaction Buffer Pack

• 产品编号：B9005S      品牌：New England Biolabs       原厂货号：B9005S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
10X ThermoPol II (Mg-free) Reaction Buffer and supplemental MgSO₄ are optimized for use with Vent_R® and Deep Vent_R™ DNA Polymerases. This buffer and supplement also provide superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. The Mg-free reaction buffer and supplemental MgSO₄ are provided for users that require complete control over Mg²⁺ concentration to optimize their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

Reaction Conditions:
At a 1X concentration, the reaction buffer, supplemented with 2 mM MgSO₄ assures optimal activity of Vent_R, Deep Vent_R, Taq and other DNA polymerases, as well as other enzymes for which ThermoPol Reaction Buffer is recommended.

Reagents Supplied:
10X ThermoPol^® II (Mg-free) Reaction Buffer
MgSO₄ (100 mM)

1X ThermoPol^® II (Mg-free) Reaction Buffer:
10 mM KCl
10 mM (NH₄)₂SO₄
20 mM Tris-HCl
0.1 % Triton X-100
pH 8.8 @ 25°C
 

原厂资料：