Description: Diluent B is recommended for making dilutions of New England Biolabs, Inc. restriction enzymes.
When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Restriction enzymes should be stored at their selling concentrations.
Reagents Supplied: 1X Diluent B
1X Diluent B:
300 mM NaCl
10 mM Tris-HCl
1 mM Dithiothreitol
0.1 mM EDTA
500 µg/ml BSA
50 % Glycerol pH 7.4 @ 25°C
原厂资料:
注意事项:
These enzyme diluent buffers are not for use with VentR® DNA Polymerases. For diluting VentR® DNA Polymerases, use (NEB #B8004S).