Red Loading Buffer Pack

• 产品编号：B7709S      品牌：New England Biolabs       原厂货号：B7709S
• 产品分类：生化试剂 > 生物缓冲液 > 蛋白生化分析缓冲液 > 蛋白电泳缓冲液
• 应用分类：

描述：

Description:
Red Loading Buffer Reagents for SDS-PAGE:

3X Red Loading Buffer (8 ml):
187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) phenol red. (Store at room temperature)

30X Reducing Agent (1 ml): 1.25 M DTT (Store at -20°C)

Notes：

General notes:

1. Reagents stored at different temperatures.

Usage notes:

1. Prepare fresh 3X Reducing Red Loading Buffer by adding 1/10 volume 30X Reducing Agent to 1 volume of 3X Red Loading Buffer.
2. Prepare samples by adding 2 volumes of Sample to 1 volume 3X Reducing Red Loading Buffer (from step 1).
3. Heat samples to 95-100°C for 3-5 minutes.
4. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobiity, load an equal volume of 1X Reducing Blue Loading Buffer into any unused wells.

原厂资料：