Blue Loading Buffer Pack

产品编号：B7703S 品牌：New England Biolabs 原厂货号：B7703S
产品分类：生化试剂 > 生物缓冲液 > 蛋白生化分析缓冲液 > 蛋白电泳缓冲液
应用分类：

包装：

运保温度:

-20°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Description:
Blue Loading Buffer Reagents for SDS-PAGE:

3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature)

30X Reducing Agent (1 ml): 1.25 M DTT (Store at -20°C)

注意事项：

General notes:

Reagents stored at different temperatures.

Usage notes:

Prepare fresh 3X Reducing Blue Loading Buffer by adding 1/10 volume 30X Reducing Agent to 1 volume of 3X Blue Loading Buffer.
Prepare samples by adding 2 volumes of Sample to 1 volume 3X Reducing Blue Loading Buffer (from step 1).
Heat samples to 95-100°C for 3-5 minutes.
After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobiity, load an equal volume of 1X Reducing Blue Loading Buffer into any unused wells.