Gel Loading Dye, Orange (6X)

• 产品编号：B7022S      品牌：New England Biolabs       原厂货号：B7022S
• 产品分类：生化试剂 > 生物缓冲液 > 核酸电泳缓冲液 > 加样缓冲液（loading buffer）
• 应用分类：

描述：

Description:
Gel Loading Dye, Orange (6X) is a pre-mixed loading buffer with a tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to their DNA substrates following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Orange G will not appear in gel photographs, and runs ahead of all but the smallest restriction fragments. Orange G migrates at approximately 50 bp on a standard 1% TBE agarose gel.

Reagents Supplied:
Gel Loading Dye, Orange (6X)

1X Gel Loading Dye, Orange (6X):
2.5 % Ficoll 400
11 mM EDTA
3.3 mM Tris-HCl
0.017 % SDS
0.15 % Orange G
pH 8.0 @ 25°C
 

原厂资料：

注意事项：

Usage notes:

1. Use 5 µl of Gel Loading Dye, Orange (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.