NEBuffer Set 1,2,3,4 & BSA

• 产品编号：B7000S      品牌：New England Biolabs       原厂货号：B7000S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
 

为确保内切酶发挥 100% 的酶切活性，NEB公司随酶免费提供 10X 反应缓冲液 (NEBuffer) 。绝大多数内切酶配有四种标准反应缓冲液中的一种，但有些内切酶配有特殊的反应缓冲液。四种标准反应缓冲液具有不同颜色标记，以便实验者使用方便。

有些内切酶需要终浓度为 100 μg/ml 的牛血清白蛋白 (BSA) ，以发挥其最大酶切活性。在这种情况下 BSA 是以 100 倍的浓度，10 mg/ml 贮存液形式提供的。对于那些不需要 BSA 即可达到最佳活性的内切酶，在反应中加入 BSA，对其活性不会造成影响。
Kit Components:
NEBuffer 1 (10X)
NEBuffer 2 (10X)
NEBuffer 3 (10X)
NEBuffer 4 (10X)
BSA (100X)

NEBuffer 1 (黄) ：
10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM dithiothreitol
(pH 7.0 @ 25℃)。
NEBuffer 2 (蓝) ：
50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM dithiothreitol
(pH 7.9 @ 25℃)。
NEBuffer 3 (红) ：
100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM
dithiothreitol (pH 7.9 @ 25℃)。
NEBuffer 4 (绿) ：
50 mM potassium acetate, 20 mM Tris-acetate, 10 mM
magnesium acetate, 1 mM dithiothreitol (pH 7.9 @ 25℃)。

原厂资料：

注意事项：

注意事项：使用前，反应缓冲液 (NEBuffer) 应彻底融化并混匀。

 New England Biolabs provides a color-coded 10X NEBuffer with each restriction enzyme to ensure (100%) optimal activity. The buffer should be used at 1X concentration in the reaction. Some restriction enzymes require bovine serum albumin (BSA) at a final concentration of 100 μg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture. Restriction enzymes that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction.

The buffer must be completely thawed before use. Dilute the 10X stock with dH₂O to a final concentration of 1X. Add the water first, buffer next, BSA if required, the DNA solution and finally the enzyme. A typical 50 µl reaction should contain 5 µl of 10X NEBuffer with the rest of the volume coming from the DNA solution, enzyme and dH₂O.