Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 μl per 1X105 cells in a 100 μl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application. * Pacific BlueTM has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific BlueTM conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
原厂资料:
Recommended Usage:
Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 μl per 1X105 cells in a 100 μl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application. * Pacific BlueTM has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific BlueTM conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.