Biotin-X, SSE reacts efficiently and reproducibly with amines. The biotin is well separated from the point of attachment by a spacer consisting of a seven-atom aminohexanoyl group (X). This spacer enhances the accessibility of biotin to the relatively deep biotin-binding sites of avidin and streptavidin. The sulfonate group on the succinimidyl ester increases the water solubility of the reactive moiety.
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