Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl antibody per 5 ml antibody dilution buffer for each mini-gel. If primary CD4+CD25+ cells are used, it is recommended that 0.5-1 x 106 cell equivalents be loaded per lane. For immunohistochemical staining of paraffin-embedded sections, the suggested use of this reagent is 1:20 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
原厂资料:
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl antibody per 5 ml antibody dilution buffer for each mini-gel. If primary CD4+CD25+ cells are used, it is recommended that 0.5-1 x 106 cell equivalents be loaded per lane. For immunohistochemical staining of paraffin-embedded sections, the suggested use of this reagent is 1:20 dilution. It is recommended that the reagent be titrated for optimal performance for each application.