Each lot of this antibody is quality control tested by Western blotting or immunofluorescence microscopy. For Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel for Western blotting. For immunofluorescence microscopy: Use a starting dilution of 1:500. Because the peptide immunogen is closely related to Aurora B, cross-reactivity to Aurora B may be observed under some experimental conditions. To minimize such cross-reactivity in immunofluorescence microscopy, it is recommended that the reagent be titrated and more stringent wash conditions be employed. It is strongly recommended that immunoprecipitation with a pan-specific Aurora antibody be carried out before Western blotting with the phospho-specific antibody to increase signal.
原厂资料：
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting or immunofluorescence microscopy. For Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel for Western blotting. For immunofluorescence microscopy: Use a starting dilution of 1:500. Because the peptide immunogen is closely related to Aurora B, cross-reactivity to Aurora B may be observed under some experimental conditions. To minimize such cross-reactivity in immunofluorescence microscopy, it is recommended that the reagent be titrated and more stringent wash conditions be employed. It is strongly recommended that immunoprecipitation with a pan-specific Aurora antibody be carried out before Western blotting with the phospho-specific antibody to increase signal.
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