Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of protein kinases, including Abl Protein Tyrosine Kinase (Abl) (NEB# P6050), CDK1-cyclin B (NEB# P6020), CaMKII (NEB #P6060), p42 MAP Kinase (MAPK) (NEB# P6080).
Reagents Supplied: 10X NEBuffer for Protein Kinases (PK)
1X NEBuffer for Protein Kinases (PK):
50 mM Tris-HCl
10 mM MgCl2
0.1 mM EDTA
2 mM DTT
0.01 % Brij 35 pH 7.5 @ 25°C