Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. To obtain a linear standard curve, serial dilutions of IFN-g recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (£ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
原厂资料：
Recommended Usage:
Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. To obtain a linear standard curve, serial dilutions of IFN-g recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (£ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
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