BioLegend’s ELISA MAX™ Deluxe Sets contain the components necessary for the accurate quantification of natural and recombinant mouse IgE. These sets are designed for cost-effective and accurate quantification of mouse IgE in cell culture supernatant, serum, plasma or other biological fluids. They are sensitive, accurate, and robust.
BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A mouse IgE specific monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IgE binds to the immobilized capture antibody. Next, a biotinylated anti-mouse IgE detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IgE present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
原厂资料：
Introduction:
BioLegend’s ELISA MAX™ Deluxe Sets contain the components necessary for the accurate quantification of natural and recombinant mouse IgE. These sets are designed for cost-effective and accurate quantification of mouse IgE in cell culture supernatant, serum, plasma or other biological fluids. They are sensitive, accurate, and robust.
BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A mouse IgE specific monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IgE binds to the immobilized capture antibody. Next, a biotinylated anti-mouse IgE detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IgE present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
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