BioLegend’s ELISA MAX™ Deluxe Sets contain the components necessary for the accurate quantification of natural and recombinant mouse IL-12/ IL-23 (p40). These sets are designed for cost-effective and accurate quantification of mouse IL-12/IL-23 (p40) in cell culture supernatant, serum, plasma or other biological fluids. They are sensitive, accurate, and robust.
IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. IL-23 is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated macrophages and dendritic cells. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. IL-12/IL-23 p40 monomer is secreted in vast excess of IL-12 p70 heterodimer; IL-12/IL-23 p40 monomer has no activity and is antagonistic to IL-12 p70 with only 1/10th the activity of p40 homodimer. The antibodies in the mouse IL-12/IL-23 (p40) ELISA MAXTM Set react with the p40 subunit of mouse IL-12 and IL-23, as monomer and homodimer.
原厂资料:
Introduction:
BioLegend’s ELISA MAX™ Deluxe Sets contain the components necessary for the accurate quantification of natural and recombinant mouse IL-12/ IL-23 (p40). These sets are designed for cost-effective and accurate quantification of mouse IL-12/IL-23 (p40) in cell culture supernatant, serum, plasma or other biological fluids. They are sensitive, accurate, and robust.
IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. IL-23 is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated macrophages and dendritic cells. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. IL-12/IL-23 p40 monomer is secreted in vast excess of IL-12 p70 heterodimer; IL-12/IL-23 p40 monomer has no activity and is antagonistic to IL-12 p70 with only 1/10th the activity of p40 homodimer. The antibodies in the mouse IL-12/IL-23 (p40) ELISA MAXTM Set react with the p40 subunit of mouse IL-12 and IL-23, as monomer and homodimer.