The bis-pyrenyl phospholipase substrate, 1,2-bis-(1-pyrenebutanoyl)-sn-glycero-3-phosphocholine emits at ~470 nm, indicating that the adjacent pyrene fluorohopres are forming excited-state dimers. Phospholipase A-mediated hydrolysis separates the fluorophores, which then emit as monomers at ~380 nm. This can be used as a substrate for either phospholipase A1 or A2.
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For Research Use Only. Not for use in diagnostic procedures.