Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer, supplemented with 1 mM MnCl2 assures optimal activity of PP1 (NEB #P0754) and Lambda Protein Phosphatase (Lambda PP NEB #P0753). NEBuffer for PMP is suitable for other members of the metallophosphatases, after addition of an appropriate metal cation.
Reagents Supplied: NEBuffer for PMP MnCl2 (10X)
1X NEBuffer for PMP:
50 mM HEPES
100 mM NaCl
2 mM DTT
0.01 % Brij 35 pH 7.5 @ 25°C