NEBuffer PI-SceI

• 产品编号：B0696S      品牌：New England Biolabs       原厂货号：B0696S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. Some restriction endonucleases require BSA at a final concentration of 100 µg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture.
Restriction endonucleases that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction mixture.

Reagents Supplied:
10X NEBuffer PI-SceI
BSA (100X)

1X NEBuffer PI-SceI:
10 mM MgCl₂
1 mM Dithiothreitol
10 mM Tris-HCl
100 mM KCl
pH 8.6 @ 25°C
 

16-Hour Incubation:
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
 

原厂资料：

注意事项：

注意事项：使用前，反应缓冲液 (NEBuffer) 应彻底融化并混匀。