RNA Loading Dye (2X)

• 产品编号：B0363S      品牌：New England Biolabs       原厂货号：B0363S
• 产品分类：生化试剂 > 生物缓冲液 > 核酸电泳缓冲液 > 加样缓冲液（loading buffer）
• 应用分类：

描述：

Description:
The RNA Loading Dye, (2X) is a premixed loading dye for use with denaturing and non-denaturing PAGE/agarose gels.

Reagents Supplied:
RNA Loading Dye, (2X)

1X RNA Loading Dye, (2X):
0.01 % SDS
0.01 % Bromophenol Blue
0.005 % Xylene Cyanol
0.05 mM EDTA
47.5 % Formamide
 

RNase Assay:
Incubation of a 10 μl reaction containing 5 µl of 2X RNA Loading Dye with 40 ng of 300 base RNA transcript for 16 hours at 37°C resulted in no detectable degradation of RNA as determined by denaturing PAGE analysis.

Endonuclease Assay:
Incubation of a 10 µl reaction containing 5 µl of 2X RNA Loading Dye with 300 ng of supercoiled plasmid for 16 hours at 37°C produced less than 10% nicked or linear molecules as determined by agarose gel electrophoresis.
 

Protocol

1. Add sample to an equal volume of RNA Loading Dye, (2X). Mix well.
2. Heat at 65–70°C for 5–10 minutes to denature RNA.
3. While heating the samples, setup the gel box and flush urea out of the wells with running buffer using a large tip.
4. Load samples.
   
   For Non-denaturing PAGE/Agarose Gel:
   Use protocol described above, but do not heat the samples

原厂资料：

注意事项：

Usage notes:

1. RNA Loading Dye, (2X) is conveniently supplied in 4 tubes. The dye can be stored at room temperature for a week, at 4°C for a month and at -20°C for 2 years.
2. The dye can also be used as a stop solution for enzyme reactions. After mixing, the samples can be stored at -20°C for at least 3 days before gel analysis.