Exonuclease I Reaction Buffer

产品编号：B0293S 品牌：New England Biolabs 原厂货号：B0293S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Reagents Supplied:
10X Exonuclease I Reaction Buffer

1X Exonuclease I Reaction Buffer:
67 mM Glycine-KOH
6.7 mM MgCl₂
10 mM 2-Mercaptoethanol
pH 9.5 @ 25°C

16-Hour Incubation:
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.