M-MuLV Reverse Transcriptase Reaction Buffer

• 产品编号：B0253S      品牌：New England Biolabs       原厂货号：B0253S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.


Reagents Supplied:
10X M-MuLV Reverse Transcriptase Reaction Buffer

1X M-MuLV Reverse Transcriptase Reaction Buffer:
50 mM Tris-HCl
75 mM KCl
3 mM MgCl₂
10 mM Dithiothreitol
pH 8.3 @ 25°C

Quality Control for Current Lot
16-Hour Incubation:
1 µg of HaeIII digested ΦX174 RF I DNA in 50µl of this reaction buffer at 1X concentration showed no detectable non-specific nuclease degradation after incubation at 37°C for 16 hours.

RNase Activity:
Incubation of 40 ng of RNA transcripts in 50 μl of this reaction buffer at a 1X concentration for 2 hours at 37°C resulted in no detectable degradation of the RNA as determined by gel electrophoresis.

Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 μg ϕX174 RF I DNA for 4 hours at 37°C in a 50 μl reaction resulted in less than 5% conversion to RF II.
 

原厂资料：

注意事项：

The buffer must be completely thawed before use. Dilute the 10X stock with dH₂O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 µl reaction should contain 5 µl of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH₂O.