Mung Bean Nuclease Reaction Buffer

产品编号：B0250S 品牌：New England Biolabs 原厂货号：B0250S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
应用分类：

包装：

6.0 ml

运保温度:

-20°C

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描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Reagents Supplied:
10X Mung Bean Nuclease Reaction Buffer

1X Mung Bean Nuclease Reaction Buffer:
50 mM sodium acetate
30 mM NaCl
1 mM ZnSO₄
pH 5.0 @ 25°C

Quality Control for Current Lot

16-Hour Incubation:
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.

注意事项：

The buffer must be completely thawed before use. Dilute the 10X stock with dH₂O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 µl reaction should contain 5 µl of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH₂O.