E. coli DNA Ligase Reaction Buffer

• 产品编号：B0205S      品牌：New England Biolabs       原厂货号：B0205S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description: 
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. 


Reagents Supplied: 
10X E. coli DNA Ligase Reaction Buffer

1X E. coli DNA Ligase Reaction Buffer:
30 mM Tris-HCl
4 mM MgCl₂
1 mM Dithiothreitol
26 µM NAD
50 µg/ml BSA
pH 8.0 @ 25°C

16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.

原厂资料：

注意事项：

注意事项：使用前，反应缓冲液（NEBuffers）应彻底融化并混匀。