Product Description
This advanced digoxigenin labeling reagent contains an embedded bis-aryl hydrazone structure,
which forms the compound’s UV-traceable chromophore; after incorporation, digoxigenin is rapidly
quantified using a non-destructive UV-scan (220-400 nm). This traceable signature enables the
nondestructive and direct quantification of digoxigenin attached to the antibody
Introduction to ChromaLink™ Labeling Technology
Sulfo ChromaLink Digoxigenin (DIG) incorporates UV-traceable DIG onto proteins containing lysine residues (amine groups) via a water-soluble-succinimidyl activated ester. Sulfo ChromaLink DIG has been engineered to include many novel features. As illustrated in Figure 1,the molecule’s structure contains a bis-aryl hydrazone chromophore (a), linked by a PEG3 linker arm (b), to digoxigenin (c). This reagent permits direct spectroscopic quantification of incorporated digoxigenin. The extended PEG3 linker preserves DIG/anti-DIG antibody affinity and maintains protein solubility after modification while the succinimidyl ester functional group (d), efficiently modifies lysines in aqueous buffers.
Application
Traceable incorporation of digoxigenin into antibodies. Linker possesses a UV-traceable
chromophore allowing for spectroscopic quantification of digoxigenin incorporation.
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