Thermo Scientific Accell siRNA Delivery Media is an enriched, serum-free media specially formulated and optimized for Thermo Scientific Accell siRNA delivery. This product is the result of thorough testing of numerous formulations, and consistently shows strong delivery, knockdown, and high cell viability when used with Accell siRNA.
While not required for use with Accell siRNA, this media provides appropriate serum-free conditions for Accell uptake while maintaining cell health. It may be replaced with another serum-free or low-serum media, or supplemented with additives known to be necessary for cell health.
Highlights
Validated in over 30 cell types for successful Accell siRNA application
Maintains cell health while providing recommended serum-free application conditions
Can be customized to your cell type with additives necessary for cell viability
Experimental Considerations
There are many cell-type specific considerations when working in low or no-serum conditions. Successful Accell siRNA results have been reported with a number of alternative conditions:
Supplemental serum up to 2.5% (high serum conditions will interfere with Accell uptake)
Growth factors and other additives (e.g. B27, insulin, glucose) necessary for cell health
Standard cell culture media formulations (with reduced serum)
Serum-free formulations (e.g. Neurobasal media)
We encourage testing of supplements or conditions that are known to benefit your particular cell type. Review the Accell Delivery Protocol for additional details.
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