Total Protein Cell Lysis Buffer is a nonionic detergent lysis buffer that enables efficient solubilization of the plasma and intracellular membranes, breaks weak intermolecular bonds, and solubilizes most proteins. Total Protein Cell Lysis Buffer reduces protein denaturation, protein complex disruption and loss of enzymatic activity since it contains less detergent than a typical RIPA lysis buffer. Simply, resuspend cells in Total Protein Cell Lysis Buffer and vortex vigoursly. Incubate the cell suspension at 4 ºC with shaking for 30 minutes. Vortex vigoursly for 30 seconds and pellet cellular debris. The supernatant contains the total protein lysate