Overview
• Only 5 minutes for PVDF or nitrocellulose blocking
• Reduce background and cross-reactivity
• Enhance chemiluminescent signals
• Protein-free formulation
Detailed Description
AMRESCO’s RapidBlock™ Solution reduces blocking time to 5 minutes for Western and Dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimizes cross-reactivity and non-specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio. Results with RapidBlock™ meet or exceed those obtained with buffers containing dried milk or BSA, which both require 1 hour of blocking time. Transfer buffers containing alcohol can increase background levels when using RapidBlock™, so alcohol-free transfer buffers, such as AMRESCO’s Rapid Transfer Buffer, should be substituted for best results. PVDF membranes can be moistened in methanol prior to use without increasing background.
Dilute stock solution with dH2O to prepare 1X RapidBlock™ just prior to use.