APO001 is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) flow cytometry assay kit, which enables the identification of cells during the late stages of apoptosis, through the labelling of DNA breaks (DNA fragmentation) and total cellular DNA.
The minimum requirement for the use of this kit is a two color flow cytometer with green fluorescence (515nm) and red fluorescence (635nm) detectors.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL
Please refer to individual component labels.
This product should be stored undiluted.
Shelf Life
12 months from date of despatch.
Instructions For Use
A full detailed protocol, describing the method for measuring apoptosis in the positive and negative control cells, and in the cell specimens, can be found atwww.abdserotec.com/uploads/IFU/APO001.pdf
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