BULK, 5X MESSAGEAMP II 100RXN(2 PTS)

产品编号：AMB17515 品牌：invitrogen 原厂货号：AMB17515
产品分类：克隆 > 体外转录 > 体外转录
应用分类：

包装：

(2 PTS)

运保温度:

store at -20°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

The MessageAmp™ II aRNA Amplification Kit amplifies as little as 100 pg of input RNA for GeneChip® analysis. Advantages of the MessageAmp™ II aRNA Amplification Kit:

• Increased amplification—up to 5,000-fold
• Greater sensitivity due to increased yields of full-length cDNA
• GeneChip® analysis with as little as 100 ng input RNA
• Efficient labeling produces high percent present calls and low 3'/5' ratios
• Includes ArrayScript™ RT, a specifically engineered M-MLV reverse transcriptase
• Optimized second strand cDNA synthesis reaction

System overview
Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide/scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA polymerase-driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp™ II aRNA Amplification Kit.

An improved kit for cDNA amplification
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The use of ArrayScript™ RT in conjunction with the optimization of the second-strand cDNA synthesis reaction results in increased conversion of mRNA into full-length, double-stranded cDNA templates. In addition, since the in vitro transcription (IVT) uses MEGAscript® technology, the IVT incubation times are shortened. These improvements in the MessageAmp™ II kit also carry through to two-round reactions, facilitating robust amplification of total RNA from samples as low as 100 pg.

Accessory products
Bio-11-UTP (Cat. No. AM8450) and Bio-16-UTP (Cat. No. AM8451) can be used in MessageAmp™ aRNA amplification reactions for target labeling for GeneChip® analysis. The Bio-UTPs are confirmed free of nonspecific endonuclease activity, exonuclease activity, and RNase activity, and are functionally tested using the MessageAmp™ II aRNA Amplification Kit.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.