Ambion® RNAlater® Tissue Collection: RNA Stabilization Solution is an aqueous, nontoxic tissue storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA.
• Single reagent immediately inactivates RNases and stabilizes RNA within tissues or cells
• No need to freeze samples in liquid nitrogen or rush samples back to the lab freezer
• Eliminates the need to freeze and grind most tissue samples
• Perfect for tissue collection "in the field"
• RNA is stable for 1 day at 37°C, 1 week at 25°C, 1 month at 4°C or indefinitely at -20°C
• Compatible with many RNA isolation procedures including most RNA isolation kits
RNAlater® stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater® for storage without jeopardizing the quality or 查看更多quantity of RNA obtained after subsequent RNA isolation. RNAlater® can be added to cell pellets and even cells in medium. The samples can then be stored frozen or unfrozen.
RNAlater® Use
The dissected tissue (less than 0.5 cm in any one dimension) is simply submerged in approximately 5 volumes of RNAlater® at room temperature. The solution permeates the cells, stabilizing the RNA. The sample can then be stored indefinitely at -20°C (the tissue does not freeze), at 4°C for up to a month or at 25°C for up to a week. For RNA isolation, the tissue is simply removed from RNAlater® and treated as though it had just been harvested. Most tissues can be transferred directly to a lysis buffer and homogenized. Samples treated with RNAlater® and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated. Cells can be spun out of RNAlater® and then added to lysis buffer, or in some cases, RNAlater® can be added along with the cells directly to the lysis buffer.
Compatible with a Variety of Procedures
RNAlater® is compatible with one-step RNA isolation methods, such as TRIzol® Reagent; with glass binding methods such as Qiagen's RNeasy™ or Ambion® RNAqueous®; with acid phenol extraction methods such as Ambion® ToTALLY RNA™; and with methods that use oligo(dT) selection of mRNA, such as Ambion® Poly(A)Purist™.
RNAlater® Applications
RNAlater® has been tested on a variety of mammalian tissues, plants, E. coli, Xenopus, fish, and Drosophila. RNAlater is ideal for:
• Protecting RNA integrity in tissues rich in RNases
• Collecting samples from different time points without having to process the samples from each time point immediately
• Archiving tissues for future microdissection
• Submerging animal cavities or organs in RNAlater® to stabilize RNA during long, tedious dissections
• Collecting samples at locations (e.g., hospitals, field sites, the space shuttle) where immediate RNA isolation is not possible
• Shipping samples on wet ice or even at room temperature if shipped overnight
In-house research, as well recently published independent research, indicates that the use of RNAlater® for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods
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