This high-purity Ambion® enzyme is a mix of cloned RNA polymerase and RNase inhibitor. It has a high specificity for its promoter, transcribing large amounts of RNA with no cross talk between promoters. It is supplied in one tube containing 1000 U at a concentration of 20 U/ µL. The enzyme incorporates radiolabeled nucleotides along with many modified nucleotides. Inclusion of RNase inhibitor provides broad-spectrum protection against enzymatic degradation of the synthesized RNA, making the enzyme ideal for the synthesis of RNA for blot andin situhybridization probes, RPA analysis,in vitrotranslation, and antisense RNA.
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For Research Use Only. Not for use in diagnostic procedures.