Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion® RNase A, RNA-Grade cleaves 3' of single-stranded C and U residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 µL (1 µg/mL). In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.
Unit Definitions:
One unit is defined as the amount of RNase A required to yield an increase in absorbance at 286 nm of 0.0146 absorbance units per min in a 1 mL volume. Assay Conditions: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA, and 1 mM cyclic 2', 3'-CMP.
Accessory Products:
Other RNA-Grade ribonuclease available include RNase V1 (SKU# AM2275) ,which cleaves base-paired nucleotides and RNase T1 (SKU# AM2283), which cleaves 3' of single-stranded G residues. Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.
RNase A, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation⁄Inactivation Buffer, and Gel Loading Buffer II should all be stored at –20°C.
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