For the isolation of extremely high-quality RNA from bacteria without the use of harsh treatments or enzymatic digestion which may alter the gene expression profile. The Ambion® kit includes sufficient reagents for 50 purifications each from 108 to 109 cells.
• Ideal even for difficult-to-lyse Gram positive bacteria
• Yields the highest-quality bacterial total RNA
• No enzymatic pretreatment required
• DNA-free™ reagents included for RT-PCR ready RNA
Traditionally, the isolation of total RNA from bacteria has required the use of hot phenol or other such harsh techniques including enzymatic digestion. Enzymatic pretreatments are thought to alter gene expression patterns on a global scale, resulting in compromised array data. The RiboPure™-Bacteria RNA Isolation Kit includes no harsh treatments and does not alter expression profiles. RiboPure™-Bacteria combines glass bead–mediated organic disruption followed by glass filter–based RNA purification for high yields 查看更多of exceptionally pure bacterial RNA. DNase treatment can be performed with the provided DNA-free™ reagents for DNA sensitive applications such as RT-PCR.
The kit has been used to isolate high quality RNA from Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Campylobacter fetus, and Rhodobacter sphaeroides. The Kit requires the use of the Vortex Adapter (see Accessory Products).
Accessory Products:
The Kit requires the use of the Vortex Adapter (SKU #AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie® 2 and holds 12 tubes. Ambion's RNAlater® RNA Stabilization Solution (SKU #AM7020) can be used to store intact bacteria for days or weeks prior to RNA isolation without compromising the integrity of the RNA. As seen in the Figure, RNA isolated from Bacillus subtilis using the RiboPure™-Bacteria Kit was stored for a week at 4°C in RNAlater® is of very high quality compared to RNA isolated from bacteria stored in TE in the same manner. For applications where the use of mRNA is desirable, this kit can be used in conjunction with the MICROBExpress™ Bacterial mRNA Enrichment Kit (SKU #AM1905).
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