MESSAGEAMP II 20RXN (2 PART KIT)

产品编号：AM1751 品牌：invitrogen 原厂货号：AM1751
产品分类：DNA和RNA纯化 > 核酸分析 > RNA扩增
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store at -20°C

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描述：

The MessageAmp™ II aRNA Amplification Kit amplifies as little as 100 pg of input RNA for GeneChip® analysis. Advantages of the MessageAmp™ II aRNA Amplification Kit:

• Increased amplification—up to 5,000-fold
• Greater sensitivity due to increased yields of full-length cDNA
• GeneChip® analysis with as little as 100 ng input RNA
• Efficient labeling produces high percent present calls and low 3'/5' ratios
• Includes ArrayScript™ RT, a specifically engineered M-MLV reverse transcriptase
• Optimized second strand cDNA synthesis reaction

System overview
Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide/scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA polymerase-driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp™ II aRNA Amplification Kit.

An improved kit for cDNA amplification
Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The use of ArrayScript™ RT in conjunction with the optimization of the second-strand cDNA synthesis reaction results in increased conversion of mRNA into full-length, double-stranded cDNA templates. In addition, since the in vitro transcription (IVT) uses MEGAscript® technology, the IVT incubation times are shortened. These improvements in the MessageAmp™ II kit also carry through to two-round reactions, facilitating robust amplification of total RNA from samples as low as 100 pg.

Accessory products
Bio-11-UTP (Cat. No. AM8450) and Bio-16-UTP (Cat. No. AM8451) can be used in MessageAmp™ aRNA amplification reactions for target labeling for GeneChip® analysis. The Bio-UTPs are confirmed free of nonspecific endonuclease activity, exonuclease activity, and RNase activity, and are functionally tested using the MessageAmp™ II aRNA Amplification Kit.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.