For the reverse transcription of RNA. The Ambion® kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions.
• Linear quantitation of mRNA targets over 106-fold range of input RNA
• Quantitate transcripts from as little as 500 fg total RNA—1⁄20th of a single cell
• Fast, flexible RT step complete in 15 min; use reaction temperatures up to 50°C
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods
MessageSensor™ is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity (see figure). The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than 2 hours.
原厂资料：
注意事项：
10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, and hGAPDH Control Primers should be stored at –20°C. HeLa S3 Control RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.
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