A synthetic miRNA mimic for miRNA functional analysis. 5 nmol is supplied.
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments.
Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
Accessory Products:
Also available are Pre-miR™ miRNA Precursors (SKU #AM17100 and #AM17101), which are designed to mimic 查看更多endogenous precursor miRNAs. To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors. Cy™3 and FAM™ dye-labeled Pre-miR™ Negative Controls (SKU #AM17120 and #AM17121) are also available for monitoring transfection efficiency in transfection experiments using Pre-miR™ miRNA Precursors.
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