RETROSCRIPT? KIT 40 RXNS EACH

• 产品编号：AM1710      品牌：invitrogen       原厂货号：AM1710
• 产品分类：克隆 > cDNA合成，cDNA文库和文库构建 > cDNA合成
• 应用分类：核酸分离纯化与分析 > RNA纯化 > 按应用类型 > 用于RT-PCR

描述：

The RETROscript® Kit includes reagents for reverse transcription of RNA into double-stranded cDNA products. Advantages of the RETROscript® Kit:

• Amplify specific RNA targets from complex mixtures (i.e., total cellular RNA)
• Both random decamers and oligo(dT)₁₈ primers provided
• Perform either one- or two-step RT-PCR

Using the RETROscript® Kit
The RETROscript® Kit provides reagents for reverse transcription of cDNA from RNA templates. When used with SuperTaq™ thermostable DNA polymerase (available separately), the cDNA templates are efficiently amplified into double-stranded DNA products. The molony-murine leukemia virus (M-MLV) reverse transcriptase is used in the first step to extend a primer hybridized to an RNA sample. M-MLV RT has higher stability and lower intrinsic RNase H activity than AMV reverse transcriptase. Both random decamers and oligo dT are provided in the RETROscript® Kit. The kit also includes two alternative buffers for first strand synthesis. Protocols are provided for performing the reverse transcription and PCR amplification reactions sequentially (two-step) or as a single reaction.

Accessory products
SuperTaq™ Polymerase (Cat. Nos. AM2054 and AM2056) and SuperTaq™ Plus Polymerase (Cat. Nos. AM2054 and AM2056) are recommended for use with the RETROscript® Kit. RETROscript® Kit components that are available separately include dNTPS for cDNA Probe Synthesis (Cat. No. AM8200), Oligo (dT) Primer (Cat. No. AM5730G), and Random Decamers (Cat. No. AM5722G). Also available are the T7-Oligo (dT)₂₄ Promoter Primer (Cat. No. AM5710) and the T7-Oligo (dT)₂₄ Promoter Primer, Anchored (Cat. No. AM5712).

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.