收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

全长cDNA扩增试剂盒,3`-5`同时做。

 
包装: EACH
运保温度:
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

The FirstChoice® RLM-RACE Kit is designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR. This kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

Features of the FirstChoice® RLM-RACE Kit:

• From RNA to PCR product in less than a day
• Yields single, specific product from rare transcripts
• Selects 5' and/or 3' ends of true messages
• Efficient—all enzymatic reactions are optimized to ensure detection of even the rarest mRNA

RACE
Rapid amplification of cDNA ends (5'-RACE) is a polymerase chain reaction-based technique developed to facilitate the cloning of sequence from the 5'-ends of messages. The FirstChoice® RLM-RACE Kit is a major improvement to the basic RACE protocol.

Selects full-length mRNA—no rRNA, tRNA or degraded mRNA
In the FirstChoice® RLM-RACE procedure, full-length mRNAs are selected by treating total or poly(A) RNA with calf intestinal phosphatase (CIP) to remove the 5'-phosphate from all molecules which contain free 5'-phosphates (ribosomal RNA, fragmented mRNA, tRNA, and contaminating genomic DNA). Full-length mRNAs are unaffected. The RNA is then treated with tobacco acid pyrophosphatase (TAP) to remove the cap structure from the full-length mRNA leaving a 5'-monophosphate. A synthetic RNA adapter is ligated to the RNA population—only molecules containing a 5'-phosphate, the uncapped, full-length mRNAs, will accept the adapter. Random-primed, reverse transcription reactions and nested PCR are then performed to amplify the 5'-end of your specific transcript.

From RNA to PCR in less than a day
Each step of the FirstChoice® RLM-RACE procedure has been optimized, so you can complete all the enzymatic reactions and even start PCR in no more than 5 hours. The FirstChoice® RLM-RACE Kit contains reagents and enzymes to produce 5 RLM-RACE-ready cDNA preparations, in addition to primers and nested RACE adapter primers for 100 PCR reactions. Reverse transcription reagents are also included. Each kit contains control RNA and primers to test the kit's performance along with a detailed Instruction Manual. SuperTaq™ Thermostable Taq DNA Polymerase is available separately. For optimal amplification of fragments ≥1 kb, use SuperTaq-Plus. Also included are a 3' RACE Adapter and Primers for 3' RACE.


注意事项:

Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate™ GAPDH-M, and 0.5 M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.


相关图片

本产品可用于的实验

最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错