miRNA 分离试剂盒(不含苯酚)

• 产品编号：AM1561      品牌：invitrogen       原厂货号：AM1561
• 产品分类：DNA和RNA纯化 > RNA纯化 > microRNA和小RNA纯化
• 应用分类：核酸分离纯化与分析 > RNA纯化 > 按样品类型 > 培养的细胞
  　　　　　核酸分离纯化与分析 > RNA纯化 > 按样品类型 > 液体和无细胞样品
  　　　　　核酸分离纯化与分析 > RNA纯化 > 按样品类型 > 植物
  　　　　　核酸分离纯化与分析 > RNA纯化 > 按样品类型 > 组织
  　　　　　核酸分离纯化与分析 > RNA纯化 > 按样品类型 > 细菌、酵母与真菌
  　　　　　核酸分离纯化与分析 > 常用核酸纯化试剂及设备 > mirVana™ 试剂盒 (mirVana™ Kit) 系列
  　　　　　暂定分类 > 表观遗传学与非编码RNA研究 > miRNA 和非编码 RNA 分析 > miRNA 分离 > 试剂盒和分析

描述：

Ambion® mirVana™ miRNA 分离试剂盒(不含苯酚)采用快速的方法，可以从组织和细胞中分离小 RNA，如 microRNA (miRNA)、小分子干扰 RNA (siRNA) 和核内小分子 RNA (snRNA)。它是基于玻璃纤维的快速高效方法，可提取各种大小的总 RNA (从几千碱基到 10-mer)。每个试剂盒包含足够40次纯化的试剂。苯酚必须单独购买。

• 高效分离含有小RNA的总RNA

• 富集小 RNA (<200 nt)，提高下游分析的灵敏度

• 简单的 30 分钟流程

• miRNA、siRNA、shRNA和snRNA分析的理想选择

• 适用于几乎所有细胞和组织类型

• 提供无苯酚

高效回收小RNA

目前的RNA纯化依赖于有机溶剂提取，然后进行乙醇沉淀或者利用玻璃纤维滤膜或硅酸盐基质吸附核酸分子。不幸的是，前一种实验方案需要酒精沉淀，这是一种耗时又低效的回收小RNA方法；后一种实验方案（如Ambion公司的RNAqueous ® 试剂盒、Qiagen公司的RNeasy ® 试剂盒）通常会导致大量小RNA的丢失。为解决这些问题，Ambion公司研制开发了 mirVana™ miRNA 分离试剂盒，采用有机溶剂提取技术，然后利用特制的结合与洗涤溶液进行玻璃纤维滤膜纯化。该试剂盒可以从几乎所有细胞和组织类型中有效回收RNA——从大分子mRNA和rRNA到10mers。 mirVana™ miRNA 分离试剂盒最多可从40份样本10²–10⁷ 培养细胞/样本或 0.5–250 mg 组织/样本——中分离含有小RNA的总RNA。

富集小RNA

Although the mirVana™ miRNA Isolation Kit 不仅可以高效纯化大于10nt的RNA，还可以分离RNA碎片——针对小RNA中特地要富集或去除的RNA。通过 mirVana™ miRNA Isolation Kit 的富集实验方案，不超过200 nt的RNA分子能够被高效地从大分子RNA中纯化出来。由此制备的RNA高度富集了miRNA、siRNA和/或snRNA。无论进行Northern分析还是溶液杂交分析，与利用总RNA的相同Assay相比，使用 mirVana™ miRNA Isolation Kit 的实验方案富集小RNA能够减弱背景，从而提高小RNA检测的灵敏度。

原厂资料：

The Ambion® mirVana™ miRNA Isolation Kit without Phenol uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)–based method. The method isolates total RNA ranging in size from kilobases down to 10mers. Each kit contains sufficient reagents for up to 40 purifications. Phenol must be purchased separately.

• Efficient isolation of small RNA–containing total RNA
• Enrich for small RNA <200 nt to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types
• Supplied without phenol

Efficient Recovery of Small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and 查看更多inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA–containing total RNA from samples consisting of 10²–10⁷ cultured cells or 0.5–250 mg tissue.

Enrich for Small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ~200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and⁄or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

Accessory Product:
mirVana™ miRNA Isolation Kit (SKU#AM1560) is available for those who want a complete kit that includes the Acid Phenol:Chloroform.